Glyconanoparticles for colorimetric bioassays

Glyconanoparticles for colorimetric bioassays.

Carbohydrate molecules are involved in many of the cellular processes that are important for life. By combining the specific analyte targeting of carbohydrates with the multivalent structure and change of solution colour as a consequence of plasmonic interactions with the aggregation of metal nanoparticles, glyconanoparticles have been used extensively for the development of bioanalytical assay...

Bioassays for anticancer activities.

The MTT/MTS in vitro cell proliferation assay is one of the most widely used assays for evaluating preliminary anticancer activity of both synthetic derivatives and natural products and natural product extracts. The highly reliable, colorimetric based assay is readily performed on a wide range of cell lines. This assay gives an indication of whole cell cytotoxicity; however, to determine the ex...

Glyconanoparticles and their interactions with lectins

Lectin-gated, mesoporous, photofunctionalized glyconanoparticles for glutathione-responsive drug delivery.

A stimuli-responsive drug delivery system based on fluorescent, lectin-gated, mesoporous glyconanoparticles has been developed and evaluated in normal- and cancer lung epithelial cells. The gating process proved efficient, exhibiting good sealing properties in the absence of the glutathione redox trigger, avoiding premature release in normal cells. In the presence of higher levels of glutathion...

Design and Analysis for Bioassays

Definition. An assay is a form of biological experiment; but the interest lies in comparing the potencies of treatments on an agreed scale, instead of in comparing the magnitude of effects of different treatments. This makes assay different from varietal trials with plants and feeding trials with animals, or clinical trials with human beings. The experimental technique may be the same, but the ...